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Image Search Results
Journal: Oncotarget
Article Title: Identification of differentially expressed miRNAs and miRNA-targeted genes in bladder cancer
doi: 10.18632/oncotarget.24441
Figure Lengend Snippet: Differentially expressed mRNAs and miRNAs were identified according to -fold changes in expression and p -value criteria. MiRNA-targeted gene pairs were identified using miRNAs and mRNAs differentially expressed in NMIBC and MIBC versus normal tissue according to previously validated informations regarding mRNA-miRNA interactions. NMIBC, non-muscle invasive bladder cancer; MIBC, muscle invasive bladder cancer.
Article Snippet: Total RNA (100 ng) from each sample was dephosphorylated, 3' end–labeled with Cy3-pCp, purified on Micro Bio-Spin columns, dried, and hybridized using the
Techniques: Expressing
Journal: Oncotarget
Article Title: Identification of differentially expressed miRNAs and miRNA-targeted genes in bladder cancer
doi: 10.18632/oncotarget.24441
Figure Lengend Snippet: (A) mRNA expression. (B) miRNA expression. BC, bladder cancer; NMIBC, non-muscle invasive bladder cancer; MIBC, muscle invasive bladder cancer.
Article Snippet: Total RNA (100 ng) from each sample was dephosphorylated, 3' end–labeled with Cy3-pCp, purified on Micro Bio-Spin columns, dried, and hybridized using the
Techniques: Expressing
Journal: Oncotarget
Article Title: Identification of differentially expressed miRNAs and miRNA-targeted genes in bladder cancer
doi: 10.18632/oncotarget.24441
Figure Lengend Snippet: (A) Genes associated with gene-miRNA interactions. (B) miRNAs associated with gene-miRNA interactions. BC, bladder cancer; NMIBC, non-muscle invasive bladder cancer; MIBC, muscle invasive bladder cancer.
Article Snippet: Total RNA (100 ng) from each sample was dephosphorylated, 3' end–labeled with Cy3-pCp, purified on Micro Bio-Spin columns, dried, and hybridized using the
Techniques:
Journal: Oncotarget
Article Title: Identification of differentially expressed miRNAs and miRNA-targeted genes in bladder cancer
doi: 10.18632/oncotarget.24441
Figure Lengend Snippet: Expression profiling of miRNA-mRNA interacted target genes in NMIBC and MIBC
Article Snippet: Total RNA (100 ng) from each sample was dephosphorylated, 3' end–labeled with Cy3-pCp, purified on Micro Bio-Spin columns, dried, and hybridized using the
Techniques: Expressing, Targeted Gene Expression
Journal: Analytical Cellular Pathology (Amsterdam)
Article Title: Hypoxia Enhances Activity and Malignant Behaviors of Colorectal Cancer Cells through the STAT3/MicroRNA-19a/PTEN/PI3K/AKT Axis
doi: 10.1155/2021/4132488
Figure Lengend Snippet: miR-19a is upregulated in the hypoxia-exposed CRC cells: (a) differentially expressed miRNAs between CRC cells cultured under hypoxic and normoxic conditions screened using a microarray analysis; (b) expression of miR-19a in HCT116 and SW480 cells cultured under hypoxic and normoxic conditions validated by RT-qPCR ( ∗ p < 0.05, two-way ANOVA); (c) expression of miR-19a in cancer and paracancerous tissues from CRC patients examined by RT-qPCR ( ∗ p < 0.05, paired t -test); (d) relevance of miR-19a expression to the survival of patients ( ∗ p < 0.05, Kaplan-Meier analysis); (e) relevance of miR-19a expression to the clinical characteristics of patients; (f) expression of miR-19a in HCT116 and SW480 and in normal NCM460 cells determined by RT-qPCR ( ∗ p < 0.05, one-way ANOVA). Data were presented as the mean ± SD from three independent experiments.
Article Snippet: The isolated miRNAs were labeled with Hy3 using a
Techniques: Cell Culture, Microarray, Expressing, Quantitative RT-PCR